Short communication: Characterization of Shiga toxin 2-carrying bacteriophages induced from Shiga-toxigenic Escherichia coli isolated from Italian dairy products

Volponi, Giorgio, Rooks, David, Smith, Darren, Picozzi, Claudia, Allison, Heather, Vigentini, Iorenzo, Foschino, Roberto and McCarthy, Alan (2012) Short communication: Characterization of Shiga toxin 2-carrying bacteriophages induced from Shiga-toxigenic Escherichia coli isolated from Italian dairy products. Journal of Dairy Science, 95 (12). pp. 6949-6956. ISSN 0022-0302

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Official URL: http://dx.doi.org/10.3168/jds.2012-5831

Abstract

Forty samples of raw milk cheese and 25 samples of raw milk itself were subjected to enrichment culture for Shiga-toxigenic Escherichia coli (STEC), and a single Shiga toxin 2- (Stx2) positive strain was obtained from one of the cheese samples. Thus, aged cheeses in which the curd is subsequently heat treated (48°C) cannot be presumed to be STEC free. Infective Stx2 bacteriophages were induced from 3 STEC strains isolated elsewhere from raw milk and 1 STEC strain from aged cheese sourced in Italy. Data on E. coli host range, morphology, genome size, and genetic variation determined by restriction fragment length polymorphism and multi-locus genotyping are presented. Although all 4 bacteriophages were found to be short-tailed Podoviridae, they exhibited considerable variation in both genome size and content. This extended to the Stx2 genes themselves, whose sequences contained several point mutations, but these did not translate to amino acid substitutions.

Item Type: Article
Uncontrolled Keywords: Shiga-toxigenic Escherichia coli, milk and cheese, Shiga toxin bacteriophage, protected designation of origin dairy product
Subjects: B400 Nutrition
C500 Microbiology
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Ay Okpokam
Date Deposited: 10 Apr 2013 08:43
Last Modified: 10 Aug 2015 11:37
URI: http://nrl.northumbria.ac.uk/id/eprint/12063

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