A Drosophila Chromatin Factor Interacts With the Piwi-Interacting RNA Mechanism in Niche Cells to Regulate Germline Stem Cell Self-Renewal

Smulders-Srinivasan, Tora, Szakmary, Akos and Lin, Haifan (2010) A Drosophila Chromatin Factor Interacts With the Piwi-Interacting RNA Mechanism in Niche Cells to Regulate Germline Stem Cell Self-Renewal. Genetics, 186 (2). pp. 573-583. ISSN 0016-6731

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Official URL: http://dx.doi.org/10.1534/genetics.110.119081

Abstract

Stem cell research has been focused on niche signaling and epigenetic programming of stem cells. However, epigenetic programming of niche cells remains unexplored. We showed previously that Piwi plays a crucial role in Piwi-interacting RNA-mediated epigenetic regulation and functions in the niche cells to maintain germline stem cells (GSCs) in the Drosophila ovary. To investigate the epigenetic programming of niche cells by Piwi, we screened mutations in the Polycomb and trithorax group genes, and an enhancer of Polycomb and trithorax called corto, for their potential genetic interaction with piwi. corto encodes a chromatin protein. corto mutations restored GSC division in mutants of piwi and fs(1)Yb (Yb), a gene that regulates piwi expression in niche cells to maintain GSCs. Consistent with this, corto appears to be expressed in the niche cells and is not required in the germline. Furthermore, in corto-suppressed Yb mutants, the expression of hedgehog (hh) is restored in niche cells, which is likely responsible for corto suppression of the GSC and somatic stem cell defects of Yb mutants. These results reveal a novel epigenetic mechanism involving Corto and Piwi that defines the fate and signaling function of niche cells in maintaining GSCs.

Item Type: Article
Subjects: C100 Biology
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Ellen Cole
Date Deposited: 26 Jul 2013 11:04
Last Modified: 24 Oct 2017 11:26
URI: http://nrl.northumbria.ac.uk/id/eprint/13277

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