Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype

Kingsley, Robert, Msefula, Chisomo, Thomson, Nicholas, Kariuki, Samuel, Holt, Kathryn, Gordon, Melita, Harris, David, Clarke, Louise, Whitehead, Sally, Sangal, Vartul, Marsh, Kevin, Achtman, Mark, Molyneux, Malcolm, Cormican, Martin, Parkhill, Julian, MacLennan, Calman, Heyderman, Robert and Dougan, Gordon (2009) Epidemic multiple drug resistant Salmonella Typhimurium causing invasive disease in sub-Saharan Africa have a distinct genotype. Genome Research, 19 (12). pp. 2279-2287. ISSN 1088-9051

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Official URL: http://dx.doi.org/10.1101/gr.091017.109

Abstract

Whereas most nontyphoidal Salmonella (NTS) are associated with gastroenteritis, there has been a dramatic increase in reports of NTS-associated invasive disease in sub-Saharan Africa. Salmonella enterica serovar Typhimurium isolates are responsible for a significant proportion of the reported invasive NTS in this region. Multilocus sequence analysis of invasive S. Typhimurium from Malawi and Kenya identified a dominant type, designated ST313, which currently is rarely reported outside of Africa. Whole-genome sequencing of a multiple drug resistant (MDR) ST313 NTS isolate, D23580, identified a distinct prophage repertoire and a composite genetic element encoding MDR genes located on a virulence-associated plasmid. Further, there was evidence of genome degradation, including pseudogene formation and chromosomal deletions, when compared with other S. Typhimurium genome sequences. Some of this genome degradation involved genes previously implicated in virulence of S. Typhimurium or genes for which the orthologs in S. Typhi are either pseudogenes or are absent. Genome analysis of other epidemic ST313 isolates from Malawi and Kenya provided evidence for microevolution and clonal replacement in the field.

Item Type: Article
Subjects: C500 Microbiology
C900 Others in Biological Sciences
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Ay Okpokam
Date Deposited: 06 Sep 2013 09:18
Last Modified: 29 Jan 2016 18:41
URI: http://nrl.northumbria.ac.uk/id/eprint/13456

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