Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone

Malik, Vatsala, Zhang, Meng, Dover, Lynn, Northen, Julian, Flinn, Anthony, Perry, Justin and Black, Gary (2013) Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone. Molecular BioSystems, 9 (11). pp. 2816-2822. ISSN 1742-206X

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Official URL: http://dx.doi.org/10.1039/C3MB70303G

Abstract

The main objectives of this work were to characterise a range of purified recombinant sterol 3β-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3β-glucosyltransferase sequence space can result in a range of enzyme selectivities. In our study the catalytically active domain of the Saccharomyces cerevisiae 3β-glucosyltransferase was used to mine putative sterol 3β-glucosyltransferases from the databases. Selected diverse sequences were expressed in and purified from Escherichia coli and shown to have different selectivities for the 3β-hydroxysteroids ergosterol and cholesterol. Surprisingly, three enzymes were also selective for testosterone, a 17β-hydroxysteroid. This study therefore reports for the first time sterol 3β-glucosyltransferases with selectivity for both 3β- and 17β-hydroxysteroids and is also the first report of recombinant 3β-glucosyltransferases with selectivity for steroids with a hydroxyl group at positions other than C-3. These enzymes could therefore find utility in the pharmaceutical industry for the green synthesis of a range of glycosylated compounds of medicinal interest.

Item Type: Article
Subjects: C900 Others in Biological Sciences
F100 Chemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Ay Okpokam
Date Deposited: 09 Oct 2013 10:50
Last Modified: 31 Jan 2016 13:00
URI: http://nrl.northumbria.ac.uk/id/eprint/13847

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