The Small GTPase Rab4A interacts with the central region of Cytoplasmic Dynein Light Intermediate Chain-1

Thornqvist, Per-Ove, Bielli, Anna, Hendrick, Alan, Finn, Robert, Fitzgerald, Kathleen and McCaffrey, Mary (2001) The Small GTPase Rab4A interacts with the central region of Cytoplasmic Dynein Light Intermediate Chain-1. Biochemical and Biophysical Research Communications, 281 (5). pp. 1141-1153. ISSN 1090-2104

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Official URL: http://dx.doi.org/10.1006/bbrc.2001.4468

Abstract

Rab4 belongs to the Rab family of small GTPases involved in the regulation of intracellular transport, and has been localized to early endosomes. We have employed the yeast two-hybrid system to identify proteins that specifically interact with Rab4AQ67L, a GTPase-deficient mutant form of Rab4A. Screening a mouse embryo cDNA library identified a clone (M449) that interacted with Rab4A in a nucleotide-dependent fashion. Data base searches identified this clone as the mouse cytoplasmic dynein light intermediate chain-1 (LIC-1). Based on this finding, the full-length equivalent human cytoplasmic dynein LIC-1 was isolated by PCR. When Rab4A was overexpressed together with either M449 or dynein LIC-1 in HeLa cells, the proteins were found to colocalize in the perinuclear region. We characterize the localization of both overexpressed human dynein LIC-1 and the endogenous protein with respect to microtubules and show that it concentrates to the microtubule-organizing center and mitotic spindle. Additionally, GFPRab4A endosomes localize to microtubules and are redistributed by nocodazole treatment. This is the first described interaction between cytoplasmic dynein, a retrograde motor protein, and a Rab protein.

Item Type: Article
Subjects: C900 Others in Biological Sciences
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Nicola King
Date Deposited: 07 Nov 2013 14:49
Last Modified: 24 Oct 2017 11:28
URI: http://nrl.northumbria.ac.uk/id/eprint/14501

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