Draft genomes of twelve host adapted and environmental isolates of Pseudomonas aeruginosa and their position in the core genome phylogeny.

Stewart, Lewis, Ford, Amy, Sangal, Vartul, Jeukens, Julie, Boyle, Brian, Caim, Shabhonam, Crossman, Lisa, Hoskisson, Paul, Levesque, Roger and Tucker, Nicholas (2014) Draft genomes of twelve host adapted and environmental isolates of Pseudomonas aeruginosa and their position in the core genome phylogeny. Pathogens and disease, 71 (1). pp. 20-25. ISSN 2049-632X

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Official URL: http://dx.doi.org/10.1111/2049-632X.12107

Abstract

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen particularly associated with the inherited disease cystic fibrosis (CF). P. aeruginosa is well known to have a large and adaptable genome that enables it to colonise a wide range of ecological niches. Here we have used a comparative genomics approach to identify changes that occur during infection of the CF lung. We used the mucoid phenotype as an obvious marker of host adaptation and compared these genomes in order to analyse SNPs, indels and islands within near-isogenic pairs. In order to commence the correction of the natural bias towards clinical isolates in genomics studies and to widen our understanding of the genomic diversity of P. aeruginosa, we included four environmental isolates in our analysis. Our data suggests that genome plasticity plays an important role in chronic infection and that the strains sequenced in this study are representative of the two major phylogenetic groups as determined by core genome SNP analysis.

Item Type: Article
Additional Information: Published online before print.
Uncontrolled Keywords: Pseudomonas aeruginosa, cystic fibrosis, genomics, core genome, phylogeny, host adaptation
Subjects: C500 Microbiology
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Vartul Sangal
Date Deposited: 08 Nov 2013 16:29
Last Modified: 10 Aug 2015 11:27
URI: http://nrl.northumbria.ac.uk/id/eprint/14525

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