Bacteria detection based on the evolution of enzyme-generated volatile organic compounds: Determination of Listeria monocytogenes in milk samples

Tait, Emma, Perry, John, Stanforth, Stephen and Dean, John (2014) Bacteria detection based on the evolution of enzyme-generated volatile organic compounds: Determination of Listeria monocytogenes in milk samples. Analytica Chimica Acta, 848. pp. 80-87. ISSN 0003-2670

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Official URL: http://dx.doi.org/10.1016/j.aca.2014.07.029

Abstract

The rapid detection of Listeria monocytogenes contamination in food is essential to prevent food-borne illness in humans. The aim of this study was to differentiate non-contaminated milk from milk contaminated with L. monocytogenes using enzyme substrates coupled with the analysis of volatile organic compounds (VOCs). The method is based on the activity of β-glucosidase and hippuricase enzymes and the detection of a specific VOC i.e. 2-nitrophenol and 3-fluoroaniline, respectively. VOCs were extracted, separated and detected by headspace-solid phase microextraction coupled to gas chromatography–mass spectrometry (HS-SPME GC–MS). This approach required the inclusion of the selective agent's cycloheximide, nalidixic acid and acriflavine HCl in the growth medium to inhibit interfering bacteria. The VOCs were liberated by L. monocytogenes provided that samples contained at least 1–1.5 × 102 CFU ml−1 of milk prior to overnight incubation. This approach shows potential for future development as a rapid method for the detection of L. monocytogenes contaminated milk.

Item Type: Article
Uncontrolled Keywords: Listeria monocytogenes, enzyme substrates, food, headspace solid-phase microextraction gas chromatography–mass spectrometry, volatile organic compounds
Subjects: F100 Chemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 12 Aug 2014 08:14
Last Modified: 24 Oct 2017 19:17
URI: http://nrl.northumbria.ac.uk/id/eprint/17390

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