Determination of the growth promoter, 4-hydroxy-3-nitrophenyl-arsonic acid in chicken tissue by coupled high-performance liquid chromatography-inductively coupled plasma mass spectrometry

Dean, John, Ebdon, Les, Foulkes, Michael, Crews, Helen and Massey, Robert (1994) Determination of the growth promoter, 4-hydroxy-3-nitrophenyl-arsonic acid in chicken tissue by coupled high-performance liquid chromatography-inductively coupled plasma mass spectrometry. Journal of Analytical Atomic Spectrometry, 9 (5). pp. 615-618. ISSN 0267 9477

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Official URL: http://dx.doi.org/10.1039/JA9940900615

Abstract

A method has been developed to measure the levels of the growth promoter 4-hydroxy-3-nitrophenylarsonic acid (roxarsone) in samples of tissue from chickens fed on a diet supplemented with this compound. Extracts of the tissue were prepared for analysis by a trypsin enzymolysis digestion technique and bulk matrix separation was performed by anion-exchange column chromatography. Further separation of the roxarsone from other species was achieved using reversed-phase high-performance liquid chromatography. Detection of the arsenical compound was by inductively coupled plasma mass spectrometry. No roxarsone was detected in chickens fed on the supplemented diet with or without a withdrawal period (7 d on base feed after the administration period) at a quantification limit of 25 ng g-1. Recoveries for roxarsone from spiked (standard additions) samples of chicken tissue varied between 85 and 103%.

Item Type: Article
Uncontrolled Keywords: 4-hydroxy-3-nitrophenylarsonic acid, arsenic speciation, chicken tissue, food analysis, high-performance liquid chromatography, inductively coupled plasma mass spectrometry
Subjects: F100 Chemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 18 Feb 2015 11:35
Last Modified: 10 Aug 2015 11:53
URI: http://nrl.northumbria.ac.uk/id/eprint/18580

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