Determination of antifungals in rodent diet by supercritical fluid extraction followed by packed column supercritical fluid chromatography with ultraviolet detection

Dean, John, Fowlis, Ian, Hitchen, Steven, Khundker, Sharmin, Ludkin, Edwin, Normand, Florence and Jones, Peter (1997) Determination of antifungals in rodent diet by supercritical fluid extraction followed by packed column supercritical fluid chromatography with ultraviolet detection. Journal of AOAC International, 80 (1). pp. 7-13. ISSN 1060-3271

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Abstract

Supercritical fluid extraction (SFE) followed by packed column supercritical fluid chromatography with ultraviolet detection was evaluated as a quantitative method for determining 4 antifungals (fluconazole, tioconazole, hexaconazole, and UK-47,265) in rodent diet. Chromatography was achieved with a cyano-bonded silica column, UV detection at 210 nm, and methanol-modified supercritical carbon dioxide as mobile phase. The effects of modifier concentration, temperature, and column pressure on antifungal retention time was studied. Off-line SFE was optimized at 2 spike levels, ranging from 0.5 to 10 g/kg, for each of the 4 antifungals. Average recoveries ranged from 79.0% for UK-47,265 to 96.5% for hexaconazole. Overall, the procedure provides a suitable method for analyzing antifungals in spiked rodent diet.

Item Type: Article
Subjects: D300 Animal Science
F100 Chemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 17 Feb 2015 10:31
Last Modified: 04 Mar 2016 15:46
URI: http://nrl.northumbria.ac.uk/id/eprint/18911

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