Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis

Taylor, Edward, Charnock, Simon, Colby, John, Davies, Gideon and Black, Gary (2001) Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis. Acta Crystallographica Section D: Biological Crystallography, 57 (8). pp. 1138-1140. ISSN 0907 4449

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Abstract

The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 Å using an in-house Cu Kα source and belong to either space group P6522 or P6122, with unit-cell parameters a = b = 72.4, c = 241.2 Å, and have two molecules of YckF in the asymmetric unit.

Item Type:	Article
Uncontrolled Keywords:	carbon fixation, 6-phospho-3-hexulose isomerase
Subjects:	C500 Microbiology
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Becky Skoyles
Date Deposited:	13 Feb 2015 11:25
Last Modified:	12 Oct 2019 17:29
URI:	http://nrl.northumbria.ac.uk/id/eprint/19274

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