A high-throughput screening method for determining the substrate scope of nitrilases

Black, Gary, Brown, Nicola, Perry, Justin, Randall, P. David, Turnbull, Graeme and Zhang, Meng (2015) A high-throughput screening method for determining the substrate scope of nitrilases. Chemical Communications, 51. pp. 2660-2662. ISSN 1359-7345

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Official URL: http://dx.doi.org/10.1039/C4CC06021K

Abstract

Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.

Item Type: Article
Subjects: B200 Pharmacology, Toxicology and Pharmacy
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Paul Burns
Date Deposited: 19 Jan 2015 16:15
Last Modified: 09 May 2017 13:15
URI: http://nrl.northumbria.ac.uk/id/eprint/21188

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