Replication, expression and segregation of plasmid-borne DNA in genetically transformed malaria parasites

van Dijk, Melissa, Vinkenoog, Rinke, Ramesar, Jai, Vervenne, Richard, Waters, Andrew and Janse, Chris (1997) Replication, expression and segregation of plasmid-borne DNA in genetically transformed malaria parasites. Molecular and Biochemical Parasitology, 86 (2). pp. 155-162. ISSN 0166 6851

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Official URL: http://dx.doi.org/10.1016/S0166-6851(97)02843-0

Abstract

To fully exploit the transfection technology developed for Plasmodium we investigated the features of replication, expression and segregation of an episomally maintained DNA construct during asexual blood stage development in genetically transformed parasites of P. berghei. Using DNA in situ hybridisation techniques we were able to show that the introduced DNA construct is located in the nucleus of the parasite and is not segregating uniformly during schizogony. Replication of the construct mainly takes place between 16 and 24 h after invasion of the merozoites, coinciding with chromosomal replication. Furthermore the plasmid-borne DHFR/TS gene is constitutively transcribed throughout the asexual blood stage development. Hence the DHFR/TS promoter would appear to be a useful tool in the study of (over)expression of introduced genes and performing complementation studies in transfected parasites during the complete asexual blood stage development of P. berghei.

Item Type: Article
Uncontrolled Keywords: Plasmodium; Transfection; Episomal; Transcription; Replication; Segregation
Subjects: C100 Biology
C400 Genetics
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 09 Apr 2015 14:51
Last Modified: 24 Oct 2017 11:28
URI: http://nrl.northumbria.ac.uk/id/eprint/22016

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