Extraction of single-copy nuclear DNA from forensic specimens with a variety of postmortem histories

Evison, Martin, Smillie, D. M. and Chamberlain, Andrew (1997) Extraction of single-copy nuclear DNA from forensic specimens with a variety of postmortem histories. Journal of Forensic Sciences, 42 (6). pp. 653-661. ISSN 0022-1198

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Abstract

Specimens of human bone. tceth and dried hlood spots frol11 3 months to 91 years old, with a variety of postmortem histories. were used in a comparative study of recovery of inglecopy nuclear DNA sequences from forensic material. Sequences of the amelogenin and HLA·DPB I genes were chosen for their value in sexing and identification. Sequences of the mitochondrial non·coding region V were also amplified to compare the recovery of mitochondrial and single·copy nuclear DNA. A variation of the silica method for DNA extraction was refined for application to the forensic specimens in this sample. Single·copy nuclear DNA was amplified from 100% of recent postoperative bone specimens (n =6), 80% of forensic teeth and bone pecimens (n = 10), 78% of recently extracted teeth (n = 18), 78% of exhumed bone up to 91 years old (n = 37) and 69% of 15 year old bone specimens fixed in 10% fomlalin (n = 20). Amelogenin sexing was correc in 85% of cases (n = 74) in which the sex of the donor had been recorded. There was no correlation between the age of the specimen and the extent of DNA preservation.

Item Type: Article
Subjects: F400 Forensic and Archaeological Science
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Martin Evison
Date Deposited: 08 May 2015 14:41
Last Modified: 10 Aug 2015 11:53
URI: http://nrl.northumbria.ac.uk/id/eprint/22358

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