Comparative analysis of virtual screening approaches in the search for novel EphA2 receptor antagonists

Callegari, Donatella, Pala, Daniela, Scalvini, Laura, Tognolini, Massimiliano, Incerti, Matteo, Rivara, Silvia, Mor, Marco and Lodola, Alessio (2015) Comparative analysis of virtual screening approaches in the search for novel EphA2 receptor antagonists. Molecules, 20 (9). pp. 17132-17151. ISSN 1420-3049

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Official URL: http://dx.doi.org/10.3390/molecules200917132

Abstract

The EphA2 receptor and its ephrin-A1 ligand form a key cell communication system, which has been found overexpressed in many cancer types and involved in tumor growth. Recent medicinal chemistry efforts have identified bile acid derivatives as low micromolar binders of the EphA2 receptor. However, these compounds suffer from poor physicochemical properties, hampering their use in vivo. The identification of compounds able to disrupt the EphA2-ephrin-A1 complex lacking the bile acid scaffold may lead to new pharmacological tools suitable for in vivo studies. To identify the most promising virtual screening (VS) protocol aimed at finding novel EphA2 antagonists, we investigated the ability of both ligand-based and structure-based approaches to retrieve known EphA2 antagonists from libraries of decoys with similar molecular properties. While ligand-based VSs were conducted using UniPR129 and ephrin-A1 ligand as reference structures, structure-based VSs were performed with Glide, using the X-ray structure of the EphA2 receptor/ephrin-A1 complex. A comparison of enrichment factors showed that ligand-based approaches outperformed the structure-based ones, suggesting ligand-based methods using the G-H loop of ephrin-A1 ligand as template as the most promising protocols to search for novel EphA2 antagonists.

Item Type: Article
Uncontrolled Keywords: Docking, drug design, EphA2 antagonist, pharmacophore search, PPI inhibitors, shape screening, UniPR129, virtual screening
Subjects: C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Nicola King
Date Deposited: 09 Dec 2015 14:39
Last Modified: 04 Nov 2016 15:42
URI: http://nrl.northumbria.ac.uk/id/eprint/24959

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