Apatone® induces endometrioid ovarian carcinoma (MDAH 2774) cells to undergo karyolysis and cell death by autoschizis: A potent and safe anticancer treatment

Gilloteaux, Jacques, Lau, H. Lee, Gourari, Ioulia, Neal, Deborah, Jamison, James M. and Summers, J.L. (2015) Apatone® induces endometrioid ovarian carcinoma (MDAH 2774) cells to undergo karyolysis and cell death by autoschizis: A potent and safe anticancer treatment. Translational Research in Anatomy, 1. pp. 25-39. ISSN 2214-854X

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Official URL: http://dx.doi.org/10.1016/j.tria.2015.10.005

Abstract

Ovarian cancers are still the most lethal gynecologic malignancy. As a novel strategy against this poor outcome cytotoxic alterations induced by a pro-oxidant treatment on human ovarian endometrioid carcinoma (MDAH 2774) cells are revisited by using light, scanning and transmission electron microscopy. A series of sequential and concomitant cellular and organelle injuries induced by ascorbate: menadione combination (VC: VK3) or Apatone® is emphasized. This adjuvant or treatment is able to kill majority of these tumor cells through ‘autoschizic cell death’, a mode of cell death different than apoptosis. Autoschizic cell death is significant after a short period of treatment to decrease the ovarian tumor cell population through induced injuries that proceed from membranes to most organelles: karyolysis with nucleolar segregation and fragmentation, autophagy of mitochondria, lysosome and other organelles as well as cytoskeletal defects. The cytoskeletal damages are evidenced by morphology changes that included auto- or self-excised pieces of cytoplasm lacking organelles apparently facilitated by grouping of vacuolated endoplasm. These results obtained against this endometrioid ovary cell line are comforted by other studies using Apatone® against other carcinomas in vitro and in vivo. Altogether these reports support Apatone® as a new drug that can favorably be used as a novel potent, safe, and inexpensive clinical adjuvant or treatment against ovarian cancers.

Item Type: Article
Uncontrolled Keywords: Ascorbate; Menadione; Endometrioid ovarian cancer MDAH 2774; Autoschizis cell death; DNA
Subjects: A300 Clinical Medicine
B200 Pharmacology, Toxicology and Pharmacy
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 16 May 2016 11:26
Last Modified: 14 May 2017 10:00
URI: http://nrl.northumbria.ac.uk/id/eprint/26814

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