Platensimycin Activity against Mycobacterial β-Ketoacyl-ACP Synthases

Brown, Alistair, Taylor, Rebecca, Bhatt, Apoorva, Futterer, Klaus and Besra, Gurdyal (2009) Platensimycin Activity against Mycobacterial β-Ketoacyl-ACP Synthases. PLoS ONE, 4 (7). e6306. ISSN 1932-6203

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Official URL: http://dx.doi.org/10.1371/journal.pone.0006306

Abstract

Background - There is an urgent need for the discovery and development of new drugs against Mycobacterium tuberculosis, the causative agent of tuberculosis, especially due to the recent emergence of multi-drug and extensively-drug resistant strains. Herein, we have examined the susceptibility of mycobacteria to the natural product platensimycin.

Methods and Findings - We have demonstrated that platensimycin has bacteriostatic activity against the fast growing Mycobacterium smegmatis (MIC = 14 µg/ml) and against Mycobacterium tuberculosis (MIC = 12 µg/ml). Growth in the presence of paltensimycin specifically inhibited the biosynthesis of mycolic acids suggesting that the antibiotic targeted the components of the mycolate biosynthesis complex. Given the inhibitory activity of platensimycin against β-ketoacyl-ACP synthases from Staphylococcus aureus, M. tuberculosis KasA, KasB or FabH were overexpressed in M. smegmatis to establish whether these mycobacterial KAS enzymes were targets of platensimycin. In M. smegmatis overexpression of kasA or kasB increased the MIC of the strains from 14 µg/ml, to 30 and 124 µg/ml respectively. However, overexpression of fabH on did not affect the MIC. Additionally, consistent with the overexpression data, in vitro assays using purified proteins demonstrated that platensimycin inhibited Mt-KasA and Mt-KasB, but not Mt-FabH.

Significance - Our results have shown that platensimycin is active against mycobacterial KasA and KasB and is thus an exciting lead compound against M. tuberculosis and the development of new synthetic analogues.

Item Type: Article
Uncontrolled Keywords: mycobacterium tuberculosis
Subjects: A100 Pre-clinical Medicine
C500 Microbiology
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: EPrint Services
Date Deposited: 11 May 2010 08:53
Last Modified: 11 May 2017 15:14
URI: http://nrl.northumbria.ac.uk/id/eprint/2727

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