Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates

Cellier, Marie, James, Arthur, Lowe, Jonathan, Orenga, Sylvain, Perry, John, Rasul, Ari and Stanforth, Stephen (2016) Detection of l-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates. Bioorganic & Medicinal Chemistry, 24 (18). pp. 4066-4074. ISSN 0968 0896

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Official URL: http://dx.doi.org/10.1016/j.bmc.2016.06.051

Abstract

A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying l-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms.

Item Type: Article
Uncontrolled Keywords: Fluorogenic enzyme substrates; l-Alanylaminopeptidase detection; Microorganisms
Subjects: C500 Microbiology
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 07 Sep 2016 09:37
Last Modified: 27 Jun 2017 03:30
URI: http://nrl.northumbria.ac.uk/id/eprint/27652

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