Evaluation of adjuvanted recombinant urease foreffective immunization againstHelicobacter pylori

Moschos, Sterghios, Somavarapu, S., Bramwell, Vincent, Randall, L., Guy, B. and Alpar, Oya (2001) Evaluation of adjuvanted recombinant urease foreffective immunization againstHelicobacter pylori. Immunology, 104 (S1). p. 98. ISSN 1365-2567

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Abstract

Urease is one of the key enzymes involved in the establishment of infection by the stomach-colonizing pathogen Helicobacter pylori. There combinant enzyme was co-administered intranasally with a range of adjuvants and their combinations. Over 6 months, we measured a number of immunological parameters to characterize both the intensity and quality of the ensuing immune responses. From the formulations tested, chitosan and alum-based adjuvants both elicited strong and sustained humor alurease-specific immunity, with the CTB þ MDP formulation shown to be superior. Following analysis of splenocyte proliferation, marked differences were seen in the concentrations of IL-2, IL-4, IL-6 and IFN-g in the supernatants of stimulated splenocytes, with profiles corresponding to antibody titre patterns. Compared to CTB, a strong inducer of all assayed cytokines, chitosan and alum adjuvants appeared to down-regulate IL-4induction, with no detectable IFN-g. In combination with CTB, MDP follows the same cytokine profile, albeit at rather lower levels, except forIL-2. We conclude that the CTB þ MDP combination offers significant potential with respect to urease-specific CD4þ cell induction, a process recently identified as essential for the clearance of H. pylori.

Item Type:	Article
Subjects:	C500 Microbiology
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Becky Skoyles
Date Deposited:	02 Feb 2017 15:11
Last Modified:	12 Oct 2019 17:29
URI:	http://nrl.northumbria.ac.uk/id/eprint/29441

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