Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates

Cellier, Marie, James, Arthur, Orenga, Sylvain, Perry, John, Rasul, Ari and Stanforth, Stephen (2017) Detection of L-alanylaminopeptidase activity in microorganisms using chromogenic self-immolative enzyme substrates. Bioorganic & Medicinal Chemistry Letters, 27 (10). pp. 2102-2106. ISSN 0960-894X

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Official URL: https://doi.org/10.1016/j.bmcl.2017.03.080

Abstract

Three potential chromogenic enzymatic probes, each possessing a self-immolative spacer unit, were synthesised for the purpose of detecting L-alanylaminopeptidase activity in microorganisms. An Alizarin-based probe was the most effective, allowing several species to generate strongly coloured colonies in the presence of metal ions.

Item Type: Article
Uncontrolled Keywords: Chromogenic enzyme substrates; L-alanylaminopeptidase detection; Microorganisms
Subjects: C500 Microbiology
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Paul Burns
Date Deposited: 30 Mar 2017 09:45
Last Modified: 25 Apr 2017 08:40
URI: http://nrl.northumbria.ac.uk/id/eprint/30282

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