Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis

Esmaeilishirazifard, Elham, De Vizio, Daniela, Moschos, Sterghios and Keshavarz, Tajali (2017) Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis. AMB Express, 7 (78). ISSN 2191-0855

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Quorum sensing molecules (QSMs) are involved in the regulation of complicated processes helping bacterial populations respond to changes in their cell-density. Although the QS gene cluster (comQXPA) has been identified in the genome sequence of some bacilli, the QS system B. licheniformis has not been investigated in detail, and its QSM (ComX pheromone) has not been identified. Given the importance of this antagonistic bacterium as an industrial workhorse, this study was aimed to elucidate B. licheniformis NCIMB-8874 QS. The results obtained from bioinformatics studies on the whole genome sequence of this strain confirmed the presence of essential quorum sensing-related genes. Although polymorphism was verified in three proteins of this cluster, ComQ, precursor-ComX and ComP, the transcription factor ComA was confirmed as the most conserved protein. The cell–cell communication of B. licheniformis NCIMB-8874 was investigated through further elucidation of the ComX pheromone as 13-amino acid peptide. The peptide sequence of the pheromone has been described through biochemical characterisation.

Item Type: Article
Uncontrolled Keywords: Quorum sensing, pheromone, bacillus, gene cloning, peptide purification, genome sequencing
Subjects: A100 Pre-clinical Medicine
B200 Pharmacology, Toxicology and Pharmacy
B900 Others in Subjects allied to Medicine
C100 Biology
C400 Genetics
C500 Microbiology
C700 Molecular Biology, Biophysics and Biochemistry
D400 Agriculture
D700 Agricultural Sciences
J700 Industrial Biotechnology
Department: Faculties > Health and Life Sciences > Applied Sciences
Related URLs:
Depositing User: Sterghios Moschos
Date Deposited: 19 Apr 2017 10:41
Last Modified: 31 Oct 2017 11:34

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