Effect of Hyperosmolality on β-Defensin Gene Expression by Human Corneal Epithelial Cells

Narayanan, Srihari, Manning, Jennifer, Proske, Rita and McDermott, Alison (2006) Effect of Hyperosmolality on β-Defensin Gene Expression by Human Corneal Epithelial Cells. Cornea, 25 (9). pp. 1063-1068. ISSN 0277-3740

Full text not available from this repository. (Request a copy)
Official URL: https://doi.org/10.1097/01.ico.0000228785.84581.35

Abstract

Purpose: As human β-defensins (hBD) are important antimicrobial peptides at epithelial surfaces, including the ocular surface, we tested the effect of hyperosmolar conditions on the expression of these peptides by human corneal epithelial cells (HCECs).

Methods: Simian virus 40-transformed HCECs (n = 5) or primary cultured HCECs (n = 5) were treated with serum-free media or serum-free hyperosmolar (400-500 mOsm/kg) media for 24 hours or serum-free 500 mOsm/kg media for 12 to 48 hours. The effect of hyperosmolality on interleukin-1β (IL-1β)-induced hBD-2 expression was also tested. IL-6 expression was studied as a marker of IL-1β function. Expression of hBD-1, -2, and -3 and IL-6 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). The levels of active IL-1β (culture supernatants and cell lysates) and pro-IL-1β (cell lysates) were detected by enzyme-linked immunosorbent assay.

Results: HCECs constitutively expressed hBD-1 and -3 but not hBD-2. Hyperosmolar media had no effect on the basal expression of hBD-1 or -3 and did not induce the expression of hBD-2. Treatment with 500 mOsm/kg media for 24 hours decreased the ability of IL-1β to upregulate hBD-2 and IL-6 expression. Active or pro-IL-1β was not detected in any cell culture sample.

Conclusion: Our results suggest that the hyperosmolar environment observed in diseases such as dry eye does not alter defensin expression. However, a hyperosmolar environment may influence cytokine function in ocular surface cells and thus affect their response to injury and inflammation.

Item Type: Article
Uncontrolled Keywords: hyperosmolality, human [beta]-defensins, corneal epithelium, cytokines
Subjects: B500 Ophthalmics
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 24 May 2017 08:39
Last Modified: 24 Oct 2017 11:27
URI: http://nrl.northumbria.ac.uk/id/eprint/30822

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics


Policies: NRL Policies | NRL University Deposit Policy | NRL Deposit Licence