Five thousand years of tropical lake sediment DNA records from Benin

Bremond, Laurent, Favier, Charly, Ficetola, Gentile Francesco, Tossou, Monique G., Akouégninou, Akpovi, Gielly, Ludovic, Giguet-Covex, Charline, Oslisly, Richard and Salzmann, Ulrich (2017) Five thousand years of tropical lake sediment DNA records from Benin. Quaternary Science Reviews, 170. pp. 203-211. ISSN 0277-3791

[img] Text (Full text)
Bremond et al.QSR 2017.pdf - Accepted Version
Restricted to Repository staff only until 8 July 2018.

Download (1MB) | Request a copy
Official URL: http://www.sciencedirect.com/science/article/pii/S...

Abstract

Until now, sedimentary DNA (sedDNA) studies have only focused on cold and temperate regions were DNA is relatively well preserved. Consequently, the tropics, where vegetation is hyperdiverse and natural archives are rare, have been neglected and deserve attention. In this study, we used next-generation sequencing to barcode sedDNA from Lake Sele, localized in the tropical lowlands of Benin (Africa), and compared the taxonomic diversity detected by DNA analyses with pollen assemblages. Plant sedDNA was successfully amplified from 33 of the 34 successfully extracted samples. In total, 43 taxa were identified along the 5,000 years spanned by the sediment: 22 taxa were identified at the family level and 21 at the genus level. The plant diversity recovered through sedDNA from Lake Sele showed a specific local signal and limited overlapping with pollen. Introduced plants, grown and cultivated close to the water, such as sweet potato, were also well recorded by sedDNA. It appears, therefore, to be a promising approach to studying past diversity in tropical regions, and could help in tracking the introduction and history of agriculture. This is the first time this method has been used in the field of domestication and dissemination of several specific crops, and the results are very encouraging.

Item Type: Article
Uncontrolled Keywords: sedDNA, metabarcoding, tropical, Africa, vegetation dynamics, plant diversity, pollen
Subjects: F800 Physical and Terrestrial Geographical and Environmental Sciences
Department: Faculties > Engineering and Environment > Geography and Environmental Sciences
Related URLs:
Depositing User: Becky Skoyles
Date Deposited: 26 Jun 2017 13:52
Last Modified: 27 Jul 2017 17:27
URI: http://nrl.northumbria.ac.uk/id/eprint/31208

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics


Policies: NRL Policies | NRL University Deposit Policy | NRL Deposit Licence