Angiopoietins 1 and 2 in sputum supernatant of optimally treated asthmatics: the effect of smoking

Petta, Vasiliki, Bakakos, Petros, Tseliou, Eleni, Kostikas, Konstantinos, Camargo Madeira Simoes, Davina, Konstantellou, Elissavet, Hillas, Georgios, Koulouris, Nikolaos, Papiris, Spyros and Loukides, Stelios (2015) Angiopoietins 1 and 2 in sputum supernatant of optimally treated asthmatics: the effect of smoking. European Journal of Clinical Investigation, 45 (1). pp. 56-62. ISSN 0014-2972

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Official URL: https://doi.org/10.1111/eci.12379

Abstract

Background - Angiopoietin-1 (Ang-1) is an essential mediator of angiogenesis by establishing vascular integrity, whereas angiopoietin-2 (Ang-2) acts as its natural inhibitor.
Objective

We aimed to determine the levels of angiopoietins in sputum supernatants of patients with optimally treated asthma and to investigate whether smoking represents a significant covariate on the above possible processes.

Methods - Eighty-seven patients with asthma (42 smokers) and 28 healthy subjects (14 smokers) were studied. All subjects underwent lung function tests, bronchial hyper-responsiveness assessment and sputum induction for cell count identification and measurement of Ang-1, Ang-2, vascular endothelial growth factor, TGF-β1, MMP-2, IL-13, Eosinophilic cationic protein and IL-8 in supernatants. Airway vascular permeability (AVP) index was also assessed.

Results - Ang-1 (ng/mL) levels were significantly higher in patients with asthma compared to normal subjects. Smoking significantly increased Ang-1 levels [median, interquartile ranges 24 (13–37) in smoking asthmatics vs 10 (7–14) in nonsmoking asthmatics vs 5·3 (3·7–6·5) and 4·6 (3·8–5·7) in healthy smokers and nonsmokers, respectively, P < 0·001]. Similar results were observed for Ang-2 (pg/mL) [168 (132–203) vs 124 (82–152) vs 94 (78–113) vs 100 (96–108), respectively, P < 0·001]. Regression analysis in the whole study population showed a significant negative association for Ang-1, with AVP index, and MMP-2. Smoking was a significant covariate for both Ang-1 and Ang-2 in asthmatic patients.

Conclusions - Ang-1 and Ang-2 levels are upregulated in patients with optimally treated asthma. Our data support a possible role for smoking in the angiogenetic process in asthma.

Item Type: Article
Uncontrolled Keywords: Angiogenesis; angiopoietins; asthma; inflammation; smoking
Subjects: B100 Anatomy, Physiology and Pathology
Department: Faculties > Health and Life Sciences > Sport, Exercise and Rehabilitation
Depositing User: Becky Skoyles
Date Deposited: 07 Dec 2017 16:15
Last Modified: 07 Dec 2017 16:16
URI: http://nrl.northumbria.ac.uk/id/eprint/32762

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