Secreted phosphoprotein-1 directly provokes vascular leakage to foster malignant pleural effusion

Psallidas, I., Stathopoulos, G., Maniatis, N., Magkouta, S., Moschos, C., Karabela, S., Kollintza, A., Camargo Madeira Simoes, Davina, Kardara, M., Vassiliou, S., Papiris, S., Roussos, C. and Kalomenidis, I. (2013) Secreted phosphoprotein-1 directly provokes vascular leakage to foster malignant pleural effusion. Oncogene, 32 (4). pp. 528-535. ISSN 0950-9232

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Official URL: https://doi.org/10.1038/onc.2012.57

Abstract

Secreted phosphoprotein-1 (SPP1) promotes cancer cell survival and regulates tumor-associated angiogenesis and inflammation, both central to the pathogenesis of malignant pleural effusion (MPE). Here, we examined the impact of tumor- and host-derived SPP1 in MPE formation and explored the mechanisms by which the cytokine exerts its effects. We used a syngeneic murine model of lung adenocarcinoma-induced MPE. To dissect the effects of tumor- versus host-derived SPP1, we intrapleurally injected wild-type and SPP1-knockout C57/BL/6 mice with either wild-type or SPP1-deficient syngeneic lung cancer cells. We demonstrated that both tumor- and host-derived SPP1 promoted pleural fluid accumulation and tumor dissemination in a synergistic manner (P<0.001). SPP1 of host origin elicited macrophage recruitment into the cancer-affected pleural cavity and boosted tumor angiogenesis, whereas tumor-derived SPP1 curtailed cancer cell apoptosis in vivo. Moreover, the cytokine directly promoted vascular hyper-permeability independently of vascular endothelial growth factor. In addition, SPP1 of tumor and host origin differentially affected the expression of proinflammatory and angiogenic mediators in the tumor microenvironment. These results suggest that SPP1 of tumor and host origin impact distinct aspects of MPE pathobiology to synergistically promote pleural fluid formation and pleural tumor progression. SPP1 may present an attractive target of therapeutic interventions for patients with MPE.

Item Type: Article
Subjects: B100 Anatomy, Physiology and Pathology
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Sport, Exercise and Rehabilitation
Depositing User: Becky Skoyles
Date Deposited: 19 Dec 2017 09:22
Last Modified: 19 Dec 2017 09:22
URI: http://nrl.northumbria.ac.uk/id/eprint/32893

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