Ligand binding pocket of a novel Allatostatin receptor type C of stick insect, Carausius morosus

Duan Sahbaz, Burcin, Sezerman, Osman, Torun, Hamdi and Birgül Iyison, Necla (2017) Ligand binding pocket of a novel Allatostatin receptor type C of stick insect, Carausius morosus. Scientific Reports, 7. p. 41266. ISSN 2045-2322

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Official URL: https://doi.org/10.1038/srep41266

Abstract

Allatostatins (AST) are neuropeptides with variable function ranging from regulation of developmental processes to the feeding behavior in insects. They exert their effects by binding to cognate GPCRs, called Allatostatin receptors (AlstR), which emerge as promising targets for pesticide design. However, AlstRs are rarely studied. This study is the first reported structural study on AlstR-AST interaction. In this work, the first C type AlstR from the stick insect Carausius morosus (CamAlstR-C) was identified and its interaction with type C AST peptide was shown to be physically consistent with the experimental results. The proposed structure of CamAlstR-C revealed a conserved motif within the third extracellular loop, which, together with the N-terminus is essential for ligand binding. In this work, computational studies were combined with molecular and nano-scale approaches in order to introduce an unknown GPCR-ligand system. Consequently, the data obtained provided a reliable target region for future agonist/inverse agonist studies on AlstRs.

Item Type: Article
Subjects: C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User: Ay Okpokam
Date Deposited: 03 Jan 2018 10:40
Last Modified: 03 Jan 2018 10:40
URI: http://nrl.northumbria.ac.uk/id/eprint/32954

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