Genomic analyses reveal two distinct lineages of Corynebacterium ulcerans strains

Subedi, Rossy, Kolodkina, Valentina, Sutcliffe, Iain, Simpson-Louredo, Liliane, Hirata, Raphael Jr., Titov, Leonid, Mattos-Guaraldi, Ana Luiza, Burkovski, Andreas and Sangal, Vartul (2018) Genomic analyses reveal two distinct lineages of Corynebacterium ulcerans strains. New Microbes and New Infections, 25. pp. 7-13. ISSN 2052-2975

[img]
Preview
Text
1-s2.0-S2052297518300404-main.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (168kB) | Preview
[img] Text
Accepted_NMNI-D-18-00019R1.pdf - Accepted Version
Restricted to Repository staff only

Download (342kB) | Request a copy
Official URL: https://doi.org/10.1016/j.nmni.2018.05.005

Abstract

Corynebacterium ulcerans is an important zoonotic pathogen which is causing diphtheria-like disease in humans, globally. In this study, the genomes of three recently isolated C. ulcerans strains, 4940, 2590 and BRAD-2649, respectively from an asymptomatic carrier, a patient with pharyngitis and a canine host were sequenced to investigate their virulence potential. A comparative analysis was performed including the published genome sequences of 16 other C. ulcerans isolates. C. ulcerans strains belong to two lineages; 13 strains grouped together in Lineage-1 and 6 strains in Lineage-2. Consistent with the zoonotic nature of C. ulcerans infections, isolates from both, the human and canine hosts clustered in both the lineages. Most of the strains possessed spaDEF and spaBC gene clusters along with the virulence genes cpp, pld, cwlH, nanH, rpfI, tspA and vsp1. The gene encoding Shiga-like toxin was only present in one strain and 11 strains carried the tox gene encoding the diphtheria-like toxin. However, none of strains 4940, 2590 and BRAD-2649 carried any toxin genes. These strains varied in the number of prophages in their genomes, which suggests these are playing an important role in introducing diversity in C. ulcerans. The pan-genomic analyses revealed a variation in the number of membrane-associated and secreted proteins that may contribute to the variation in the pathogenicity between different strains.

Item Type: Article
Uncontrolled Keywords: Corynebacterium ulcerans, diphtheria, nontoxigenic, sore throat, toxigenic, vaccine, virulence, zoonotic
Subjects: B100 Anatomy, Physiology and Pathology
C500 Microbiology
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 21 May 2018 14:22
Last Modified: 11 Oct 2019 06:38
URI: http://nrl.northumbria.ac.uk/id/eprint/34296

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics