Determination ofNɛ-(Carboxymethyl)lysine in Foods and Related Systems

Ames, Jenny (2008) Determination ofNɛ-(Carboxymethyl)lysine in Foods and Related Systems. Annals of the New York Academy of Sciences, 1126. pp. 20-24. ISSN 0077-8923

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Official URL: http://dx.doi.org/10.1196/annals.1433.030

Abstract

The sensitive and specific determination of advanced glycation end products (AGEs) is of considerable interest because these compounds have been associated with pro-oxidative and proinflammatory effects in vivo. AGEs form when carbonyl compounds, such as glucose and its oxidation products, glyoxal and methylglyoxal, react with the ?-amino group of lysine and the guanidino group of arginine to give structures including N?-(carboxymethyl)lysine (CML), N?-(carboxyethyl)lysine, and hydroimidazolones. CML is frequently used as a marker for AGEs in general. It exists in both the free or peptide-bound forms. Analysis of CML involves its extraction from the food (including protein hydrolysis to release any peptide-bound adduct) and determination by immunochemical or instrumental means. Various factors must be considered at each step of the analysis. Extraction, hydrolysis, and sample clean-up are all less straight forward for food samples, compared to plasma and tissue. The immunochemical and instrumental methods all have their advantages and disadvantages, and no perfect method exists. Currently, different procedures are being used in different laboratories, and there is an urgent need to compare, improve, and validate methods.

Item Type: Article
Uncontrolled Keywords: Maillard reaction, Glycosylation
Subjects: B400 Nutrition
F100 Chemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: EPrints Services
Date Deposited: 11 Feb 2010 11:51
Last Modified: 10 Aug 2015 11:46
URI: http://nrl.northumbria.ac.uk/id/eprint/351

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