Interstitial cell network volume is reduced in the terminal bowel of ageing mice

Gamage, Prasanna, Patel, Bhavik, Yeoman, Mark, Ranson, Rachel and Saffrey, M. Jill (2018) Interstitial cell network volume is reduced in the terminal bowel of ageing mice. Journal of Cellular and Molecular Medicine, 22 (10). pp. 5160-5164. ISSN 1582-1838

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Official URL: http://dx.doi.org/10.1111/jcmm.13794

Abstract

Ageing is associated with impaired neuromuscular function of the terminal gastrointestinal (GI) tract, which can result in chronic constipation, faecal impaction and incontinence. Interstitial cells of cajal (ICC) play an important role in regulation of intestinal smooth muscle contraction. However, changes in ICC volume with age in the terminal GI tract (the anal canal including the anal sphincter region and rectum) have not been studied. Here, the distribution, morphology and network volume of ICC in the terminal GI tract of 3‐ to 4‐month‐old and 26‐ to 28‐month‐old C57BL/6 mice were investigated. ICC were identified by immunofluorescence labelling of wholemount preparations with an antibody against c‐Kit. ICC network volume was measured by software‐based 3D volume rendering of confocal Z stacks. A significant reduction in ICC network volume per unit volume of muscle was measured in aged animals. No age‐associated change in ICC morphology was detected. The thickness of the circular muscle layer of the anal sphincter region and rectum increased with age, while that in the distal colon decreased. These results suggest that ageing is associated with a reduction in the network volume of ICC in the terminal GI tract, which may influence the normal function of these regions.

Item Type: Article
Uncontrolled Keywords: autonomic nervous system, constipation, enteric nervous system, gastrointestinal tract, IAS, incontinence, internal anal sphincter, smooth muscle
Subjects: B100 Anatomy, Physiology and Pathology
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 15 Aug 2018 07:51
Last Modified: 11 Oct 2019 17:31
URI: http://nrl.northumbria.ac.uk/id/eprint/35335

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