Transcriptomics and proteomics show that selenium affects inflammation, cytoskeleton, and cancer pathways in human rectal biopsies

Méplan, Catherine, Johnson, Ian, Polley, Abigael, Cockell, Simon, Bradburn, David, Commane, Daniel, Arasaradnam, Ramesh, Mulholland, Francis, Zupanic, Anze, Mathers, John and Hesketh, John (2016) Transcriptomics and proteomics show that selenium affects inflammation, cytoskeleton, and cancer pathways in human rectal biopsies. The FASEB Journal, 30 (8). pp. 2812-2825. ISSN 0892-6638

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Official URL: http://dx.doi.org/10.1096/fj.201600251R

Abstract

Epidemiologic studies highlight the potential role of dietary selenium (Se) in colorectal cancer prevention. Our goal was to elucidate whether expression of factors crucial for colorectal homoeostasis is affected by physiologic differences in Se status. Using transcriptomics and proteomics followed by pathway analysis, we identified pathways affected by Se status in rectal biopsies from 22 healthy adults, including 11 controls with optimal status (mean plasma Se = 1.43 μM) and 11 subjects with suboptimal status (mean plasma Se = 0.86 μM). We observed that 254 genes and 26 proteins implicated in cancer (80%), immune function and inflammatory response (40%), cell growth and proliferation (70%), cellular movement, and cell death (50%) were differentially expressed between the 2 groups. Expression of 69 genes, including selenoproteins W1 and K, which are genes involved in cytoskeleton remodelling and transcription factor NFκB signaling, correlated significantly with Se status. Integrating proteomics and transcriptomics datasets revealed reduced inflammatory and immune responses and cytoskeleton remodelling in the suboptimal Se status group. This is the first study combining omics technologies to describe the impact of differences in Se status on colorectal expression patterns, revealing that suboptimal Se status could alter inflammatory signaling and cytoskeleton in human rectal mucosa and so influence cancer risk.

Item Type: Article
Uncontrolled Keywords: selenoprotein, NFkB, colorectal, cytokeratin, nutrigenomics
Subjects: B900 Others in Subjects allied to Medicine
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Paul Burns
Date Deposited: 23 Aug 2018 16:25
Last Modified: 11 Oct 2019 06:32
URI: http://nrl.northumbria.ac.uk/id/eprint/35462

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