Bioengineering the microanatomy of human skin

Roger, Mathilde, Fullard, Nicola, Costello, Lydia, Bradbury, Steven, Markiewicz, Ewa, O'Reilly, Steven, Darling, Nicole, Ritchie, Pamela, Määttä, Arto, Karakesisoglou, Iakowos, Nelson, Glyn, von Zglinicki, Thomas, Dicolandrea, Teresa, Isfort, Robert, Bascom, Charles and Przyborski, Stefan (2019) Bioengineering the microanatomy of human skin. Journal of Anatomy, 234 (4). pp. 438-455. ISSN 0021-8782

[img]
Preview
Text
Roger et al - Bioengineering the microanatomy of human skin OA.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (3MB) | Preview
Official URL: https://doi.org/10.1111/joa.12942

Abstract

Recreating the structure of human tissues in the laboratory is valuable for fundamental research, testing interventions, and reducing the use of animals. Critical to the use of such technology is the ability to produce tissue models that accurately reproduce the microanatomy of the native tissue. Current artificial cell‐based skin systems lack thorough characterisation, are not representative of human skin, and can show variation. In this study, we have developed a novel full thickness model of human skin comprised of epidermal and dermal compartments. Using an inert porous scaffold, we created a dermal construct using human fibroblasts that secrete their own extracellular matrix proteins, which avoids the use of animal‐derived materials. The dermal construct acts as a foundation upon which epidermal keratinocytes were seeded and differentiated into a stratified keratinised epithelium. In‐depth morphological analyses of the model demonstrated very close similarities with native human skin. Extensive immunostaining and electron microscopy analysis revealed ultrastructural details such as keratohyalin granules and lamellar bodies within the stratum granulosum, specialised junctional complexes, and the presence of a basal lamina. These features reflect the functional characteristics and barrier properties of the skin equivalent. Robustness and reproducibility of in vitro models are important attributes in experimental practice, and we demonstrate the consistency of the skin construct between different users. In summary, a new model of full thickness human skin has been developed that possesses microanatomical features reminiscent of native tissue. This skin model platform will be of significant interest to scientists researching the structure and function of human skin.

Item Type: Article
Uncontrolled Keywords: barrier function, dermis, epidermis, human, methodology, reproducible, skin equivalent, tissue engineering
Subjects: C100 Biology
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Paul Burns
Date Deposited: 13 Feb 2019 11:44
Last Modified: 11 Oct 2019 07:46
URI: http://nrl.northumbria.ac.uk/id/eprint/37961

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics


Policies: NRL Policies | NRL University Deposit Policy | NRL Deposit Licence