Bacterial sensors define intracellular free energies for correct enzyme metalation

Osman, Deenah, Martini, Maria Alessandra, Foster, Andrew W., Chen, Junjun, Scott, Andrew J. P., Morton, Richard J., Steed, Jonathan W., Lurie-Luke, Elena, Huggins, Thomas G., Lawrence, Andrew D., Deery, Evelyne, Warren, Martin J., Chivers, Peter T. and Robinson, Nigel J. (2019) Bacterial sensors define intracellular free energies for correct enzyme metalation. Nature Chemical Biology, 15 (3). pp. 241-249. ISSN 1552-4450

[img]
Preview
Text
Osman et al author accepted for library.pdf - Accepted Version

Download (1MB) | Preview
Official URL: https://doi.org/10.1038/s41589-018-0211-4

Abstract

There is a challenge for metalloenzymes to acquire their correct metals because some inorganic elements form more stable complexes with proteins than do others. These preferences can be overcome provided some metals are more available than others. However, while the total amount of cellular metal can be readily measured, the available levels of each metal have been more difficult to define. Metal-sensing transcriptional regulators are tuned to the intracellular availabilities of their cognate ions. Here we have determined the standard free energy for metal complex formation to which each sensor, in a set of bacterial metal sensors, is attuned: the less competitive the metal, the less favorable the free energy and hence the greater availability to which the cognate allosteric mechanism is tuned. Comparing these free energies with values derived from the metal affinities of a metalloprotein reveals the mechanism of correct metalation exemplified here by a cobalt chelatase for vitamin B 12 .

Item Type: Article
Subjects: C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User: Elena Carlaw
Date Deposited: 01 Mar 2019 13:57
Last Modified: 11 Oct 2019 10:06
URI: http://nrl.northumbria.ac.uk/id/eprint/38258

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics


Policies: NRL Policies | NRL University Deposit Policy | NRL Deposit Licence