Fluorogenic L-alanylaminopeptidase substrates derived from 6-amino-2-hetarylquinolines and 7-amino-3-hetarylcoumarins and their potential applications in diagnostic microbiology

Cellier-Rastit, Marie, James, Arthur L., Orenga, Sylvain, Perry, John D., Robinson, Shaun N., Turnbull, Graeme and Stanforth, Stephen P. (2019) Fluorogenic L-alanylaminopeptidase substrates derived from 6-amino-2-hetarylquinolines and 7-amino-3-hetarylcoumarins and their potential applications in diagnostic microbiology. Bioorganic & Medicinal Chemistry Letters, 29 (10). pp. 1227-1231. ISSN 0960-894X

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Official URL: https://doi.org/10.1016/j.bmcl.2019.03.011

Abstract

Six novel fluorogenic enzyme substrates for detecting L-alanylaminopeptidase activity in microorganisms have been prepared and evaluated in Columbia agar media. The substrates are L-alanyl derivatives of 6-amino-2-hetarylquinolines and 7-amino-3-hetarylcoumarins. Both the quinoline and coumarin series of substrates produced fluorescence in the presence of Gram-negative microorganisms. In contrast, fluorescence generation in the presence of the Gram-positive microorganisms and yeasts was limited or absent.

Item Type: Article
Uncontrolled Keywords: Fluorogenic substrates, L-alanylaminopeptidase, microorganism detection, quinolines, coumarins
Subjects: C500 Microbiology
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 13 Mar 2019 11:41
Last Modified: 11 Oct 2019 07:52
URI: http://nrl.northumbria.ac.uk/id/eprint/38383

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