Vibrational spectroscopy combined with transcriptomic analysis for investigation of bacterial responses towards acid stress

Hlaing, Mya M., Wood, Bayden R., McNaughton, Don, Rood, Julian I., Fox, Edward M. and Augustin, Mary Ann (2018) Vibrational spectroscopy combined with transcriptomic analysis for investigation of bacterial responses towards acid stress. Applied Microbiology and Biotechnology, 102 (1). pp. 333-343. ISSN 0175-7598

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Abstract

The ability of bacteria to tolerate acid stress plays an important role in their growth and survival. In particular, aciduric bacteria have several survival systems that prevent cell damage from acid stress. In this study, the effect of the bacterial stress induced by pre-adaptation at different pH values on the cellular macromolecules of Lactobacillus plantarum was investigated using Raman spectroscopy and Fourier transform infrared spectroscopy. The expression of key genes was also quantified to provide understanding of the transcriptional response of the cells to lethal acid stress conditions. Principal component analysis of the spectra exhibited marked differences in the spectral regions associated with carbohydrates, lipids, proteins, and nucleic acids for all acid-stressed cells compared to those of untreated control cells. The changes in spectroscopic and transcriptomic profiles that were observed revealed alterations in bacterial cell wall composition after acid treatment. The results suggest the existence of a complex bacterial stress response in which modifications of cellular compounds from pre-adaption at low pH are involved. This study demonstrates the potential application of vibrational spectroscopy techniques to discriminate between intact and injured bacterial cells as well as to study their stress responses after exposure to acid environments during food processing.

Item Type:	Article
Uncontrolled Keywords:	Raman spectroscopy, Fourier transform infrared spectroscopy, Acid stress response, Gene expression, Lactobacillus plantarum
Subjects:	C500 Microbiology
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Paul Burns
Date Deposited:	21 Mar 2019 18:03
Last Modified:	10 Oct 2019 21:16
URI:	http://nrl.northumbria.ac.uk/id/eprint/38491

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