Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer

Ribeiro, Ricardo, Pal, Deepali, Jamieson, David, Rankin, Kenneth, Benning, Matthew, Dalgarno, Kenneth and Ferreira, Ana (2017) Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer. ACS Applied Materials & Interfaces, 9 (15). pp. 12967-12974. ISSN 1944-8244

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Official URL: http://dx.doi.org/10.1021/acsami.6b16434

Abstract

Temporary single-cell coating is a useful tool for cell processing, allowing manipulation of cells to prevent cell attachment and agglomeration, before re-establishing normal cell function. In this work, a speckled coating method using a known polycation [poly(l-lysine), PLL] is described to induce cell surface electrostatic charges on three different cell types, namely, two bone cancer cell lines and fibroblasts. The morphology of the PLL speckled coating on the cell surface, internalization and metabolization of the polymer, and prevention of cellular aggregations are reported. Polymer concentration was found to be the key parameter controlling both capsule morphology and cell health. This approach allows a temporary cell coating over the course of 1–2 h, with cells exhibiting phenotypically normal behavior after ingesting and metabolizing the polymer. The process offers a fast and efficient alternative to aid single-cell manipulation for bioprocessing applications. Preliminary work on the application of PLL speckled cell coating in enabling reliable bioprinting is also presented.

Item Type: Article
Uncontrolled Keywords: bioprinting; bioprocessing; cellular uptake; coating; polycation; single cells; temporary
Subjects: F100 Chemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Becky Skoyles
Date Deposited: 28 Mar 2019 15:49
Last Modified: 28 Mar 2019 16:00
URI: http://nrl.northumbria.ac.uk/id/eprint/38617

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