Fluorogenic 7-azidocoumarin and 3/4-azidophthalimide derivatives as indicators of reductase activity in microorganisms

Chalansonnet, Valerie, Lowe, John, Orenga, Sylvain, Perry, John D., Robinson, Shaun N., Stanforth, Stephen, Sykes, Hannah E. and Truong, Thang V. (2019) Fluorogenic 7-azidocoumarin and 3/4-azidophthalimide derivatives as indicators of reductase activity in microorganisms. Bioorganic & Medicinal Chemistry Letters, 29 (16). pp. 2354-2357. ISSN 0960-894X

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Official URL: https://doi.org/10.1016/j.bmcl.2019.06.010

Abstract

A series of fluorogenic heterocyclic azides were prepared and assessed as reductase substrates across a selection of Gram-negative and Gram-positive microorganisms. The majority of these azides showed similar activity profiles to nitroreductase substrates. Microorganisms that do not produce hydrogen sulfide reduced the azides, indicating reductase activity was not linked to hydrogen sulfide production.A series of fluorogenic heterocyclic azides were prepared and assessed as reductase substrates across a selection of Gram-negative and Gram-positive microorganisms. The majority of these azides showed similar activity profiles to nitroreductase substrates. Microorganisms that do not produce hydrogen sulfide reduced the azides, indicating reductase activity was not linked to hydrogen sulfide production.

Item Type: Article
Uncontrolled Keywords: fluorogenic substrates, fluorogenic azides, nitroreductase, microorganism detection
Subjects: C700 Molecular Biology, Biophysics and Biochemistry
F100 Chemistry
Department: Faculties > Arts, Design and Social Sciences > Social Sciences
Depositing User: Elena Carlaw
Date Deposited: 10 Jun 2019 10:36
Last Modified: 11 Oct 2019 12:45
URI: http://nrl.northumbria.ac.uk/id/eprint/39586

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