Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778)

Lima-Barbero, José Francisco, Díaz-Sanchez, Sandra, Sparagano, Olivier, Finn, Robert, de la Fuente, José and Villar, Margarita (2019) Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778). Avian Pathology, 48 (sup1). S52-S59. ISSN 0307-9457

[img]
Preview
Text
18_09_2019_Metaproteo.pdf - Published Version
Available under License Creative Commons Attribution 4.0.

Download (2MB) | Preview
[img] Text
10_07_2019_Metaproteo (1).pdf - Accepted Version
Restricted to Repository staff only until 3 July 2020.

Download (1MB) | Request a copy
Official URL: https://doi.org/10.1080/03079457.2019.1635679

Abstract

The poultry red mite (PRM), Dermanyssus gallinae (De Geer, 1778), is a worldwide distributed ectoparasite and considered a major pest affecting the laying hen industry in Europe. Based on available information in other ectoparasites, the mite microbiome might participate in several biological processes and the acquisition, maintenance and transmission of pathogens. However, little is known about the role of PRM as a mechanical carrier or a biological vector in the transmission of pathogenic bacteria. Herein, we used a metaproteomics approach to characterize the alphaproteobacteria in the microbiota of PRM, and variations in its profile with ectoparasite development (nymphs vs. adults) and feeding (unfed vs. fed). The results showed that the bacterial community associated with D. gallinae was mainly composed of environmental and commensal bacteria. Putative symbiotic bacteria of the genera Wolbachia, C. Tokpelaia and Sphingomonas were identified, together with potential pathogenic bacteria of the genera Inquilinus, Neorickettsia and Roseomonas. Significant differences in the composition of alphaproteobacterial microbiota were associated with mite development and feeding, suggesting that bacteria have functional implications in metabolic pathways associated with blood feeding. These results support the use of metaproteomics for the characterization of alphaproteobacteria associated with the D. gallinae microbiota that could provide relevant information for the understanding of mite-host interactions and the development of potential control interventions.

Item Type: Article
Uncontrolled Keywords: Dermanyssus, microbiome, proteomics, mite, bacteria, poultry, pathogen
Subjects: B100 Anatomy, Physiology and Pathology
C500 Microbiology
D900 Others in Veterinary Sciences, Agriculture and related subjects
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 10 Jul 2019 09:38
Last Modified: 11 Oct 2019 13:15
URI: http://nrl.northumbria.ac.uk/id/eprint/39936

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics


Policies: NRL Policies | NRL University Deposit Policy | NRL Deposit Licence