A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria

Kelly, Ciarán, Taylor, George M., Hitchcock, Andrew, Torres-Méndez, Antonio and Heap, John T. (2018) A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria. ACS Synthetic Biology, 7 (4). pp. 1056-1066. ISSN 2161-5063

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Official URL: https://doi.org/10.1021/acssynbio.7b00435

Abstract

Cyanobacteria are important for fundamental studies of photosynthesis and have great biotechnological potential. In order to better study and fully exploit these organisms, the limited repertoire of genetic tools and parts must be expanded. A small number of inducible promoters have been used in cyanobacteria, allowing dynamic external control of gene expression through the addition of specific inducer molecules. However, the inducible promoters used to date suffer from various drawbacks including toxicity of inducers, leaky expression in the absence of inducer and inducer photolability, the latter being particularly relevant to cyanobacteria, which, as photoautotrophs, are grown under light. Here we introduce the rhamnose-inducible rhaBAD promoter of Escherichia coli into the model freshwater cyanobacterium Synechocystis sp. PCC 6803 and demonstrate it has superior properties to previously reported cyanobacterial inducible promoter systems, such as a non-toxic, photostable, non-metabolizable inducer, a linear response to inducer concentration and crucially no basal transcription in the absence of inducer.

Item Type: Article
Uncontrolled Keywords: Synechocystis, cyanobacteria, rhamnose, inducible promoter, gene expression, synthetic biology
Subjects: C100 Biology
C700 Molecular Biology, Biophysics and Biochemistry
F100 Chemistry
F200 Materials Science
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 25 Sep 2019 12:43
Last Modified: 21 Nov 2019 14:00
URI: http://nrl.northumbria.ac.uk/id/eprint/40844

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