Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli

Kelly, Ciarán, Pinske, Constanze, Murphy, Bonnie J., Parkin, Alison, Armstrong, Fraser, Palmer, Tracy and Sargent, Frank (2015) Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli. Biotechnology Reports, 8. pp. 94-104. ISSN 2215-017X

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Official URL: https://doi.org/10.1016/j.btre.2015.10.002

Abstract

Biohydrogen is a potentially useful product of microbial energy metabolism. One approach to engineering biohydrogen production in bacteria is the production of non-native hydrogenase activity in a host cell, for example Escherichia coli. In some microbes, hydrogenase enzymes are linked directly to central metabolism via diaphorase enzymes that utilise NAD+/NADH cofactors. In this work, it was hypothesised that heterologous production of an NAD+/NADH-linked hydrogenase could connect hydrogen production in an E. coli host directly to its central metabolism. To test this, a synthetic operon was designed and characterised encoding an apparently NADH-dependent, hydrogen-evolving [FeFe]-hydrogenase from Caldanaerobacter subterranus. The synthetic operon was stably integrated into the E. coli chromosome and shown to produce an active hydrogenase, however no H2 production was observed. Subsequently, it was found that heterologous co-production of a pyruvate::ferredoxin oxidoreductase and ferredoxin from Thermotoga maritima was found to be essential to drive H2 production by this system. This work provides genetic evidence that the Ca.subterranus [FeFe]-hydrogenase could be operating in vivo as an electron-confurcating enzyme.

Item Type: Article
Uncontrolled Keywords: Bacterial hydrogen metabolism, Fermentation, Protein engineering, Molecular genetics[FeFe]-hydrogenase, Electron-bifurcation
Subjects: C100 Biology
C700 Molecular Biology, Biophysics and Biochemistry
F100 Chemistry
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Elena Carlaw
Date Deposited: 25 Sep 2019 15:31
Last Modified: 21 Nov 2019 14:00
URI: http://nrl.northumbria.ac.uk/id/eprint/40852

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