Review of NAD(P)H-dependent oxidoreductases: Properties, engineering and application

Sellés Vidal, Lara, Kelly, Ciarán, Mordaka, Paweł M. and Heap, John T. (2018) Review of NAD(P)H-dependent oxidoreductases: Properties, engineering and application. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1866 (2). pp. 327-347. ISSN 1570-9639

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Abstract

NAD(P)H-dependent oxidoreductases catalyze the reduction or oxidation of a substrate coupled to the oxidation or reduction, respectively, of a nicotinamide adenine dinucleotide cofactor NAD(P)H or NAD(P)+. NAD(P)H-dependent oxidoreductases catalyze a large variety of reactions and play a pivotal role in many central metabolic pathways. Due to the high activity, regiospecificity and stereospecificity with which they catalyze redox reactions, they have been used as key components in a wide range of applications, including substrate utilization, the synthesis of chemicals, biodegradation and detoxification. There is great interest in tailoring NAD(P)H-dependent oxidoreductases to make them more suitable for particular applications. Here, we review the main properties and classes of NAD(P)H-dependent oxidoreductases, the types of reactions they catalyze, some of the main protein engineering techniques used to modify their properties and some interesting examples of their modification and application.

Item Type:	Article
Uncontrolled Keywords:	Animals, Humans, NADH, NADPH Oxidoreductases/chemistry, NADP/chemistry, Protein Engineering/methods
Subjects:	C700 Molecular Biology, Biophysics and Biochemistry
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Paul Burns
Date Deposited:	25 Sep 2019 16:50
Last Modified:	10 Oct 2019 14:48
URI:	http://nrl.northumbria.ac.uk/id/eprint/40859

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