Exercise intensity modulates the appearance of circulating microvesicles with proangiogenic potential upon endothelial cells

Wilhelm, Eurico N., González-Alonso, José, Parris, Christopher and Rakobowchuk, Mark (2016) Exercise intensity modulates the appearance of circulating microvesicles with proangiogenic potential upon endothelial cells. American Journal of Physiology-Heart and Circulatory Physiology, 311 (5). H1297-H1310. ISSN 0363-6135

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Exercise intensity modulates the appearance of circulating microvesicles with proangiogenic potential upon endothelial cells.pdf - Accepted Version

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Official URL: https://doi.org/10.1152/ajpheart.00516.2016

Abstract

The effect of endurance exercise on circulating microvesicle dynamics and their impact on surrounding endothelial cells is unclear. Here we tested the hypothesis that exercise intensity modulates the time course of platelet (PMV) and endothelial-derived (EMV) microvesicle appearance in the circulation through hemodynamic and biochemical-related mechanisms, and that microvesicles formed during exercise would stimulate endothelial angiogenesis in vitro. Nine healthy young men had venous blood samples taken before, during, and throughout the recovery period after 1 h of moderate [46 ± 2% maximal oxygen uptake (V̇o2max)] or heavy (67 ± 2% V̇o2max) intensity semirecumbent cycling and a time-matched resting control trial. In vitro experiments were performed by incubating endothelial cells with rest and exercise-derived microvesicles to examine their effects on cell angiogenic capacities. PMVs (CD41+) increased from baseline only during heavy exercise (from 21 ± 1 × 103 to 55 ± 8 × 103 and 48 ± 6 × 103 PMV/μl at 30 and 60 min, respectively; P < 0.05), returning to baseline early in postexercise recovery (P > 0.05), whereas EMVs (CD62E+) were unchanged (P > 0.05). PMVs were related to brachial artery shear rate (r2 = 0.43) and plasma norepinephrine concentrations (r2 = 0.21) during exercise (P < 0.05). Exercise-derived microvesicles enhanced endothelial proliferation, migration, and tubule formation compared with rest microvesicles (P < 0.05). These results demonstrate substantial increases in circulating PMVs during heavy exercise and that exercise-derived microvesicles stimulate human endothelial cells by enhancing angiogenesis and proliferation. This involvement of microvesicles may be considered a novel mechanism through which exercise mediates vascular healing and adaptation.

Item Type: Article
Uncontrolled Keywords: platelet microvesicles, microparticles, shear stress, human umbilical vein endothelial cell, angiogenesis
Subjects: B100 Anatomy, Physiology and Pathology
C600 Sports Science
Department: Faculties > Health and Life Sciences > Sport, Exercise and Rehabilitation
Depositing User: Rachel Branson
Date Deposited: 13 Mar 2020 15:43
Last Modified: 13 Mar 2020 15:43
URI: http://nrl.northumbria.ac.uk/id/eprint/42479

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