Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms

Partington, Matthew, Masic, Dino, Nicholson, Audrey, Perry, John, Berrington, Andrew, West, Jonatan and Sparagano, Olivier (2008) Toward a PCR-Independent Molecular Diagnosis of Veterinary and Medically Relevant Pathogenic Organisms. Annals of the New York Academy of Sciences, 1149 (1). pp. 391-393. ISSN 0077-8923

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Official URL: http://dx.doi.org/10.1196/annals.1428.010

Abstract

Bloodstream infections caused by bacteria and fungi are a major problem worldwide. These bloodstream infections can affect both people and livestock, placing a significant burden upon developed and developing economies. In this paper we describe a multiplexed testing format, which can identify a range of bacteria and fungi within a single blood sample. Key to this technique is the specificity and sensitivity of the nucleotide probes that capture the sample. The sensitivity and specificity of the probes may allow detection of disease-causing microorganisms without the need for polymerase chain reaction amplification if the dynamics of probe binding can be observed in real time.

Item Type: Article
Uncontrolled Keywords: aspergillus, candida, reverse line blot, PCR, diagnostics
Subjects: C100 Biology
C500 Microbiology
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Ellen Cole
Date Deposited: 12 Jan 2012 14:53
Last Modified: 10 Aug 2015 11:47
URI: http://nrl.northumbria.ac.uk/id/eprint/4534

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