Flavanol monomer-induced changes to the human faecal microflora

Tzounis, Xenofon, Vulevic, Jelena, Kuhnle, Gunter, George, Trevor, Leonczak, Jadwiga, Gibson, Glenn, Kwik-Uribe, Catherine and Spencer, Jeremy (2008) Flavanol monomer-induced changes to the human faecal microflora. British Journal of Nutrition, 99 (04). pp. 782-792. ISSN 0007-1145

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Official URL: http://dx.doi.org/10.1017/S0007114507853384

Abstract

We have investigated the bacterial-dependent metabolism of ( − )-epicatechin and (+)-catechin using a pH-controlled, stirred, batch-culture fermentation system reflective of the distal region of the human large intestine. Incubation of ( − )-epicatechin or (+)-catechin (150 mg/l or 1000 mg/l) with faecal bacteria, led to the generation of 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone, 5-phenyl-γ-valerolactone and phenylpropionic acid. However, the formation of these metabolites from (+)-catechin required its initial conversion to (+)-epicatechin. The metabolism of both flavanols occurred in the presence of favourable carbon sources, notably sucrose and the prebiotic fructo-oligosaccharides, indicating that bacterial utilisation of flavanols also occurs when preferential energy sources are available. (+)-Catechin incubation affected the growth of select microflora, resulting in a statistically significant increase in the growth of the Clostridium coccoides–Eubacterium rectale group, Bifidobacterium spp. and Escherichia coli, as well as a significant inhibitory effect on the growth of the C. histolyticum group. In contrast, the effect of ( − )-epicatechin was less profound, only significantly increasing the growth of the C. coccoides–Eubacterium rectale group. These potential prebiotic effects for both (+)-catechin and ( − )-epicatechin were most notable at the lower concentration of 150 mg/l. As both ( − )-epicatechin and (+)-catechin were converted to the same metabolites, the more dramatic change in the growth of distinct microfloral populations produced by (+)-catechin incubation may be linked to the bacterial conversion of (+)-catechin to (+)-epicatechin. Together these data suggest that the consumption of flavanol-rich foods may support gut health through their ability to exert prebiotic actions.

Item Type: Article
Uncontrolled Keywords: flavanols, prebiotics, faecal microflora, large intestine
Subjects: C900 Others in Biological Sciences
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Ay Okpokam
Date Deposited: 18 Jan 2012 10:49
Last Modified: 13 May 2017 06:09
URI: http://nrl.northumbria.ac.uk/id/eprint/4561

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