Cytoprotective effects of phenolic antioxidants and essential fatty acids in human blood monocyte and neuroblastoma cell lines: Surrogates for neurological damage in vivo

Young, Julie, Wahle, Klaus and Boyle, Susanne (2008) Cytoprotective effects of phenolic antioxidants and essential fatty acids in human blood monocyte and neuroblastoma cell lines: Surrogates for neurological damage in vivo. Prostaglandins, Leukotrienes and Essential Fatty Acids, 78 (1). pp. 45-59. ISSN 0952-3278

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Official URL: http://dx.doi.org/10.1016/j.plefa.2007.10.005

Abstract

Background
Oxidative stress is implicated in the development of a range of neurological diseases. There is increasing interest in the neuroprotective efficacy of antioxidants in modulating such processes with at least one polyphenolic being tested as a prophylactic in Alzheimer's disease. Beneficial effects of adjunctive n-3 polyunsaturated fatty acids with combined intakes of vitamin C and E on both the positive and negative symptoms of schizophrenia have been reported. Robust in vitro systems are desirable, enabling a mechanistic investigation of the molecular mechanisms underpinning such effects and identification of further potentially efficacious nutraceuticals.

Materials and method
A comparative study employing a human lymphoblastoid cell line derived from a subject with early onset schizophrenia, a neuroblastoma IMR-32 cell line and the histiocytic lymphoma U937 cell line was undertaken. The cytoprotective effects of two phenols in affording protection to cellular DNA from an oxidative challenge were assessed in untreated and fatty acid treated cell lines.

Results and conclusion
Marked differences in the uptake of fatty acids by the cell types were found and the IMR-32 cell line was most susceptible to the oxidant challenge. Hydroxytyrosol gave significant cytoprotection in all three-cell lines and this possible neuroprotective efficacy warrants further investigation, both in vitro and in vivo.

Item Type: Article
Uncontrolled Keywords: Glutathione-Peroxidase activity, independent phospholiphase A(2), central nervous system,oxidative stress, eicosapentaenoic acid, U937 cells, olive oil
Subjects: C900 Others in Biological Sciences
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Ay Okpokam
Date Deposited: 19 Jan 2012 13:45
Last Modified: 24 Oct 2017 11:27
URI: http://nrl.northumbria.ac.uk/id/eprint/4594

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