MIG (CXCL9) is a more sensitive measure than IFN-γ of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines

Berthoud, Tamara, Dunachie, Susanna, Todryk, Stephen, Hill, Adrian and Fletcher, Helen (2009) MIG (CXCL9) is a more sensitive measure than IFN-γ of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines. Journal of Immunological Methods, 340 (1). pp. 33-41. ISSN 0022-1759

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Official URL: http://dx.doi.org/10.1016/j.jim.2008.09.021

Abstract

For many years the IFN-γ ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-γ and has the potential to provide amplification of the IFN-γ signal. MIG secretion could provide a measure of bio-active IFN-γ and a functional IFN-γ signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-γ using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-γ present. Measurement of MIG alongside IFN-γ may provide a fuller picture of Th1 type responses post-vaccination.

Item Type: Article
Uncontrolled Keywords: T cells, malaria vaccine
Subjects: B900 Others in Subjects allied to Medicine
C100 Biology
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: EPrints Services
Date Deposited: 19 Apr 2010 12:05
Last Modified: 10 Aug 2015 11:44
URI: http://nrl.northumbria.ac.uk/id/eprint/534

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