Evidence For Th1/2/17 Cytokine Involvement In Pigeon Fanciers' Hypersensitivity Pneumonitis (hp)

Anderson, Kenneth, Donnelly, Iona, Jolly, Lisa, Pushparaj, Peter, Adamson, Margaret, Todryk, Stephen, Bourke, Stephen and McSharry, Charles (2011) Evidence For Th1/2/17 Cytokine Involvement In Pigeon Fanciers' Hypersensitivity Pneumonitis (hp). In: American Thoracic Society 2011 International Conference, 13-18 May 2011, Denver, CO..

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Official URL: http://www.atsjournals.org/doi/abs/10.1164/ajrccm-...

Abstract

Introduction
The immuno-pathogenesis of HP is unresolved. Serum antibody appears to be necessary but insufficient for disease. Murine models suggest that HP is primarily a disease of T-helper (h)1 lymphocyte subset function (Matsuno Y, Ishii Y, 2007) that is dependent on interleukin (IL)-12 and interferon(IFN)γ cytokine activity (Gudmundsson G, Monick MM, 1998). Recently Th17 family cytokines have also been associated with experimental HP (Simonian PL, Roark CL, 2009). The contribution of these cytokines to HP is unresolved.

Aim and Methods
In order to determine the association between Th1/2/17 and symptoms in pigeon fanciers’ HP we quantified the serum and lymphocyte production of IL-1b, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-17A/F, IL-22, TNFα, IFNγ and GM-CSF, and compared these with immunological sensitisation assessed by serum IgG and lymphocyte responses to avian antigens and with recorded symptoms after avian antigen exposure in 45 pigeon fanciers. Cytokines and antibody activity were quantified by enzyme immunoassay.

Results
Symptom categories typical of HP were identified by cluster analysis and were associated with serum antibody but not with serum cytokines. Antigen-specific lymphocyte responses were predominantly associated with in vitro production of IL-10, GM-CSF and IFNγ (p<0.005).

Conclusions
Serum antibody appears useful for confirming symptom clusters in HP. Those with HP have antigen-specific lymphocytes that produce a mixed Th1 and Th2 but not Th17 cytokine profile. These observations, requiring confirmation in alveolar lymphocytes, suggest that HP may consist of mixed clinical and immunological phenotypes.

Item Type: Conference or Workshop Item (Poster)
Subjects: B900 Others in Subjects allied to Medicine
C100 Biology
Department: Faculties > Health and Life Sciences > Applied Sciences
Depositing User: Ellen Cole
Date Deposited: 14 Feb 2012 16:28
Last Modified: 24 Oct 2017 11:26
URI: http://nrl.northumbria.ac.uk/id/eprint/5364

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