Multicomponent organic nanoparticles for fluorescence studies in biological systems

McDonald, Tom, Martin, Philip, Patterson, Joseph, Smith, Darren, Giardiello, Marco, Marcello, Marco, See, Violaine, O'Reilly, Rachel, Owen, Andrew and Rannard, Steven (2012) Multicomponent organic nanoparticles for fluorescence studies in biological systems. Advanced Functional Materials, 22 (12). pp. 2469-2478. ISSN 1616-301X

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Official URL: http://dx.doi.org/10.1002/adfm.201103059

Abstract

The formation of dual-component organic nanoparticles by a modified emulsion-templated freeze-drying approach leads to aqueous nanosuspensions showing fluorescence (Förster) resonance energy transfer (FRET) from within a distribution of single nanoparticles. The combination of both FRET dyes within dual-component nanoparticles (<200 nm) allows the spatial and physical monitoring of the particles, as the FRET signal is lost on dissolution and breakdown of the nanoparticles. The monitoring of accumulation by Caco-2 cells and macrophages shows very limited internalization within the non-phagocytic cells. Conservation of FRET within the macrophages confirms extensive whole-particle internalization. The cellular permeability through Caco-2 monolayers is also assessed and movement of intact dual-component particles is observed, suggesting a mechanism for enhanced pharmacokinetics in vivo.

Item Type: Article
Additional Information: This is the pre-peer reviewed version of the following article: McDonald, T. O., Martin, P., Patterson, J. P., Smith, D., Giardiello, M., Marcello, M., See, V., O'Reilly, R. K., Owen, A. and Rannard, S. (2012), Multicomponent Organic Nanoparticles for Fluorescence Studies in Biological Systems. Adv. Funct. Mater., 22: 2469–2478 which has been published in final form at http://dx.doi.org/10.1002/adfm.201103059
Uncontrolled Keywords: nanosuspensions, cellular uptake, fluorescence resonance energy transfer (FRET)
Subjects: C100 Biology
C700 Molecular Biology, Biophysics and Biochemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Ay Okpokam
Date Deposited: 26 Mar 2012 09:11
Last Modified: 09 May 2017 19:30
URI: http://nrl.northumbria.ac.uk/id/eprint/5861

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