Analytical bias between species caused by matrix effects in quantitative analysis of a small-molecule pharmaceutical candidate in plasma

Gray, Nicholas, McDougall, Stuart and Dean, John (2012) Analytical bias between species caused by matrix effects in quantitative analysis of a small-molecule pharmaceutical candidate in plasma. Bioanalysis, 4 (6). pp. 675-684. ISSN 1757-6180

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Official URL: http://dx.doi.org/10.4155/BIO.12.11

Abstract

Background: Suppression or enhancement of MS ionization, particularly evident when electrospray is used as the source of ions, has been widely discussed.

Methods: An assay for a small-molecule pharmaceutical in dog
plasma between 1–300 ng/ml was validated with a mean bias across the calibration range of 5.0%. When the calibration sample matrix was substituted for human plasma, the mean bias across the range increased to 29.1%. A study of bias originating as a result of matrix effects, arising from endogenous glycerophosphocholine species, in plasma sources is discussed.

Conclusion: A simple strategy to assess the potential of any unmitigated matrix effect to bias quantitative analysis by nonequivalent ionization induction or suppression is evaluated

Item Type: Article
Subjects: F100 Chemistry
Department: Faculties > Health and Life Sciences > School of Life Sciences > Applied Sciences
Depositing User: Linda Barlow
Date Deposited: 05 Apr 2012 08:29
Last Modified: 10 Aug 2015 11:36
URI: http://nrl.northumbria.ac.uk/id/eprint/6052

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