Fluorogenic substrates for the detection of microbial nitroreductases.

James, Arthur, Jay, C., Monget, D., Perry, John, Rasburn, J. W. and Gould, Frances Kate (2001) Fluorogenic substrates for the detection of microbial nitroreductases. Letters in Applied Microbiology, 33 (6). pp. 403-408. ISSN 0266-8254

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Abstract

Aims: To synthesize and evaluate fluorogenic substrates for the detection of microbial nitroreductases. These substrates, all based on 7-nitrocoumarin, may be reduced to form fluorescent aminocoumarins.
Methods and Results: Thirty pathogenic microbial strains, including both bacteria and yeasts, were examined for nitroreductase activity in a whole-cell assay. All strains readily reduced each of the seven substrates to generate fluorescence, suggesting the widespread presence of nitroreductase activity in pathogenic bacteria.
Conclusions, Significance and Impact of the Study: These novel substrates facilitate the direct detection of nitroreductase activity and have potential as sensitive indicators of microbial growth.

Item Type:	Article
Additional Information:	This paper describes the synthesis of substrates designed to detect clinically important microorganisms. Prof. James designed and synthesised the substrates. This work has been patented (James, A.; Monget, D., EP 1124986) and involved collaboration with BioMerieux
Uncontrolled Keywords:	microorganisms, detection
Subjects:	A100 Pre-clinical Medicine C500 Microbiology
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	EPrint Services
Date Deposited:	23 Dec 2008 09:23
Last Modified:	12 Oct 2019 17:29
URI:	http://nrl.northumbria.ac.uk/id/eprint/1351

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