The Small GTPase Rab4A interacts with the central region of Cytoplasmic Dynein Light Intermediate Chain-1

Thornqvist, Per-Ove, Bielli, Anna, Hendrick, Alan, Finn, Robert, Fitzgerald, Kathleen and McCaffrey, Mary (2001) The Small GTPase Rab4A interacts with the central region of Cytoplasmic Dynein Light Intermediate Chain-1. Biochemical and Biophysical Research Communications, 281 (5). pp. 1141-1153. ISSN 1090-2104

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Abstract

Rab4 belongs to the Rab family of small GTPases involved in the regulation of intracellular transport, and has been localized to early endosomes. We have employed the yeast two-hybrid system to identify proteins that specifically interact with Rab4AQ67L, a GTPase-deficient mutant form of Rab4A. Screening a mouse embryo cDNA library identified a clone (M449) that interacted with Rab4A in a nucleotide-dependent fashion. Data base searches identified this clone as the mouse cytoplasmic dynein light intermediate chain-1 (LIC-1). Based on this finding, the full-length equivalent human cytoplasmic dynein LIC-1 was isolated by PCR. When Rab4A was overexpressed together with either M449 or dynein LIC-1 in HeLa cells, the proteins were found to colocalize in the perinuclear region. We characterize the localization of both overexpressed human dynein LIC-1 and the endogenous protein with respect to microtubules and show that it concentrates to the microtubule-organizing center and mitotic spindle. Additionally, GFPRab4A endosomes localize to microtubules and are redistributed by nocodazole treatment. This is the first described interaction between cytoplasmic dynein, a retrograde motor protein, and a Rab protein.

Item Type:	Article
Subjects:	C900 Others in Biological Sciences
Department:	Faculties > Health and Life Sciences > Applied Sciences
Depositing User:	Users 6424 not found.
Date Deposited:	07 Nov 2013 14:49
Last Modified:	12 Oct 2019 17:29
URI:	http://nrl.northumbria.ac.uk/id/eprint/14501

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