Miniaturized Magnetic Bead-Actuators for Force-Clamp Spectroscopy-Based Single-Molecule Measurements

Feng, Luying and Torun, Hamdi (2020) Miniaturized Magnetic Bead-Actuators for Force-Clamp Spectroscopy-Based Single-Molecule Measurements. Ultramicroscopy, 209. p. 112888. ISSN 0304-3991

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Official URL: https://doi.org/10.1016/j.ultramic.2019.112888

Abstract

Force-clamp spectroscopy can mimic the physiological conditions for the proteins under investigation. In addition, it is a direct way of observing the relationship between bond lifetime and molecular forces. However, traditional force-clamp methods rely on active feedback controllers that can introduce artefacts. In this work, we introduce a new method to enable force-clamp spectroscopy without a need for an active feedback. The method is based on miniaturized magnetic beads offering improved stability. As a case study, we performed force-clamp experiments using biotin-streptavidin molecule pairs with and without active feedback. Our results demonstrate the feasibility of force-clamp experiments without feedback and illustrate the advantages of our method.

Item Type: Article
Uncontrolled Keywords: Atomic force microscopy, Single-Molecule Mechanics, Force-clamp Spectroscopy, Biotin, Streptavidin
Subjects: F300 Physics
H600 Electronic and Electrical Engineering
H800 Chemical, Process and Energy Engineering
Department: Faculties > Engineering and Environment > Mathematics, Physics and Electrical Engineering
Depositing User: Elena Carlaw
Date Deposited: 11 Nov 2019 13:48
Last Modified: 10 Mar 2020 15:00
URI: http://nrl.northumbria.ac.uk/id/eprint/41407

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